Download PDF by Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W.: Advances in Neurochemistry: Volume 3

By Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W. Agranoff, M. H. Aprison (eds.)

ISBN-10: 1461582407

ISBN-13: 9781461582403

ISBN-10: 1461582423

ISBN-13: 9781461582427

The unique premise of the Editors in starting up this sequence used to be that there existed a readership ofneurochemists with huge biochemical again­ floor who might utilize a sequence devoted to either new boost­ ments and really expert stories in neurochemistry. Having chosen our authors, now we have provided them almost whole freedom to mirror and speculate in a box within which they've got accomplished prominence. The reaction to the 1st volumes has been profitable. the current one keeps during this culture. whereas we've not tried to submit really expert volumes, the current quantity includes a little comparable chapters (Chapters four and five, at the position of amino acid neurotransmitters). the 1st 3 chapters learn 3 assorted ways, every one of present curiosity, in neurochemi­ cal techniques to the molecular bases of neuronal and glial constitution. B. W. Agranoff M. H. Aprison vii CONTENTS bankruptcy 1 2',3'-CYCUC NUCLEOTIDE 3'-PHOSPHODIESTERASE NEIL RAYMOND SIMS AND PATRICK ROBERT CARNEGIE 1. creation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. Assay of CNPase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three 2. 1. desire for Activation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three 2. 2. comparability of Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . three three. organization of CNPase with Myelin . . . . . . . . . . . . . . . . . . . . . . . 7 three. 1. historic. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 three. 2. Subcellular Fractionation. . . . . . . . . . . . . . . . . . . . . . . . . . . . eight three. three. improvement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . eight three. four. Mutant Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . nine three. five. Use as a Myelin Marker . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 three. 6. Myelin-Related Fractions and Peripheral Nerve Myelin. eleven four. CNPase in Nonmyelin Fractions. . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. Activation and Isolation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. 1. creation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . thirteen five. 2. Activation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 five. three. Solubilization and Fractionation. . . . . . . . . . . . . . . . . . . . . . 15 6. homes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 6. 1. Substrate Specificity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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Additional resources for Advances in Neurochemistry: Volume 3

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Vol. 1, pp. 1-29, Dekker, New York. , 1974, Cyclic nucleotide phosphodiesterase in silkworm: Purification and properties, Biochim. Biophys. Acta 370:227-241. Nakhasi, H. , Toews, A. , and Horrocks, L. , 1975, Effects of a postnatal protein deficiency on the content and composition of myelin from brains of weanling rats, Brain Res. 83:176-179. , Ittel, M. , 1974, Ribonucleases of beef brain nuclei. Purification and characterization of an alkaline RNAase, Biochim. Biophys. Acta 358:91-104. Norton, W.

1973), using 2' ,3'-cyclic CMP as substrate, obtained 50% inhibition with all three compounds at approximately half the substrate concentration. In part this probably reflects the higher affinity of the enzyme for purine compared to pyrimidine bases (2' ,3'-cyclic AMP being hydrolyzed at approximately three times the rate of 2' ,3' -cyclic CMP). 0 when inhibition was detected. 2, respectively, and since the monodissociated form more closely resembles 2' ,3'-cyclic AMP on charge considerations, it is not surprising that inhibition was more readily detectable at the lower pH.

Zimmerman, A. , Quarles, R. , Brady, R. , and Hsu, J. , 1976, Hypomyelination in copper-deficient rats: Prenatal and postnatal copper replacement, Arch. Neurol. 33:111-119. CHAPTER 2 IMMUNOHISTOCHEMISTR Y OF NERVOUS SYSTEMSPECIFIC ANTIGENS LA WRENCE F. ENG AND JOHN W. BIGBEE Department of Pathology Stanford University School of Medicine Stanford, California and Veterans Administration Hospital Palo Alto, California 1. INTRODUCTION In recent years, immunohistochemical detection and localization of specific antigens in tissue sections have had important clinical and experimental applications.

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Advances in Neurochemistry: Volume 3 by Neil Raymond Sims, Patrick Robert Carnegie (auth.), B. W. Agranoff, M. H. Aprison (eds.)


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